IL-27 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-40-678-2ug | 2ug | £307.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Blue Ice or room temp
Storage:
The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Documents
Further Information
Additional Names:
Interleukin-27, Interleukin-27 subunit alpha, IL-27-A, Interleukin-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, p28, Interleukin-30, IL30
Background:
As a member of the IL-12 family of heterodimeric cytokines that also includes IL-12, IL-23, and IL-35, IL-27 is formed by the association of an IL-27-p28 subunit (also known as IL-30) with the Epstein-Barr Virus (EBV)-induced Gene 3 (EBI3) subunit (also known as IL-27B). Expressed by antigen-presenting cells (APCs) in the early phases of antigen-mediated activation, IL-27 acts as a critical initiator of adaptive immune responses by promoting the rapid clonal expansion of na?ve CD4+ T cells, IFN-γ production, and Th1 polarization. IL-27 elicits its effects through receptor complexes IL-27R (also known as TCCR/WSX-1) and gp130, a receptor shared by IL-6. Mainly expressed in monocytes, endothelial cells, and dendritic cells, IL-27 plays an important role alongside IL-6 in the regulation of inflammation and autoimmunity; directly antagonizing IL-6?s stimulation of CD4+ T cell proliferation and Th17 differentiation. Recombinant Human IL-27 produced from HEK293 cells is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa.
Biological Activity:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml.
NCBI Gene ID #:
246778
NCBI Official Name:
interleukin 27
NCBI Official Symbol:
IL27
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Purity:
> 90% by SDS-PAGE gel and HPLC analyses.
Recombinant Protein Sequence:
#N/A
Source:
HEK293 cells
Swissprot #:
Q8NEV9