AMC Standard Solution
| Code | Size | Price |
|---|
| SBB-RB0128-R100 | 100 ul | £95.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Blue Ice
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
7-Amino-4-methylcoumarin; Coumarin 120
Appearance:
Liquid.
CAS:
26093-31-2
EClass:
32160000
Form (Short):
liquid
Formulation:
50µM stock solution in DMSO.
Handling Advice:
Avoid freeze/thaw cycles.Protect from light.
InChi:
InChI=1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
InChiKey:
GLNDAGDHSLMOKX-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 26093-31-2. Formula: C10H9NO2. MW: 175.2. Synthetic. A fluorogenic standard useful for quantitating assays monitoring amido-4-methylcoumarin (AMC) release. These include ubiquitin-AMC, SUMO-1-AMC, NEDD8-AMC, ISG15-AMC and proteasome substrates. The concentration of the AMC released can be calculated from a standard curve prepared with AMC standards.
Molecular Formula:
C10H9NO2
Molecular Weight:
175.2
Other data:
Use: Serial dilute in reaction buffer to form a standard curve within the range of sensitivity determined for the assay and plate reader. The recommend upper final concentration for AMC curves is 500 nM. The excitation and emission wavelengths of AMC are 360nm and 460nm, respectively. Other wavelengths may be used as long as measurements are consistent for both the experimental samples and the dilution curve.
Package Type:
Vial
Product Description:
A fluorogenic standard useful for quantitating assays monitoring amido-4-methylcoumarin (AMC) release. These include ubiquitin-AMC, SUMO-1-AMC, NEDD8-AMC, ISG15-AMC and proteasome substrates. The concentration of the AMC released can be calculated from a standard curve prepared with AMC standards.
SMILES:
CC1=CC(=O)OC2=C1C=CC(N)=C2
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C. Store solutions at -20°C in the dark.