AFC Standard Solution
| Code | Size | Price |
|---|
| SBB-RB0129-R100 | 100 ul | £95.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Blue Ice
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
7-Amino-4-trifluoromethylcoumarin
Appearance:
Liquid.
CAS:
53518-15-3
EClass:
32160000
Form (Short):
liquid
Formulation:
50µM stock solution in DMSO.
Handling Advice:
Avoid freeze/thaw cycles.Protect from light.
InChi:
InChI=1S/C10H6F3NO2/c11-10(12,13)7-4-9(15)16-8-3-5(14)1-2-6(7)8/h1-4H,14H2
InChiKey:
JBNOVHJXQSHGRL-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 53518-15-3. Formula: C10H6F3NO2. MW: 229.2. Synthetic. A fluorogenic standard useful for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. The concentration of the AFC released can be calculated from a standard curve prepared with AFC standards.
MDL:
MFCD00006858
Molecular Formula:
C10H6F3NO2
Molecular Weight:
229.2
Other data:
Use: Serial dilute in reaction buffer to form a standard curve within the range of sensitivity determined for the assay and plate reader. The recommend upper final concentration for AFC curves is 500 nM. The excitation and emission wavelengths of AFC are 400nm and 505nm, respectively.
Package Type:
Vial
Product Description:
A fluorogenic standard useful for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC. The concentration of the AFC released can be calculated from a standard curve prepared with AFC standards.
SMILES:
NC1=CC=C2C(OC(=O)C=C2C(F)(F)F)=C1
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C. Store solutions at -20°C in the dark.