CLIA Kit for Hepatocyte Growth Factor (HGF)
| Code | Size | Price |
|---|
| SCA047Rb-24T | 24T | £273.00 |
Quantity:
| SCA047Rb-48T | 48T | £455.00 |
Quantity:
| SCA047Rb-96T | 96T | £637.00 |
Quantity:
| SCA047Rb-96T*5 | 96T*5 | £2,760.00 |
Quantity:
| SCA047Rb-96T*10 | 96T*10 | £5,186.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rabbit
Application: Chemiluminescent Immunoassay (CLIA)
Further Information
Alternative Names:
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
Assay length:
2h, 40min
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Detection range:
15.62-1000pg/mL
Format:
48T, 96T, 96T?5, 96T?10, 96T?100
Item Name:
Hepatocyte Growth Factor
Method:
Double-antibody Sandwich
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Research Area:
Cytokine;Tumor immunity;Hepatology;
Sample type:
Serum, plasma and other biological fluids
Sensitivity:
The minimum detectable dose of this kit is typically less than 0.62pg/mL
Specificity:
This assay has high sensitivity and excellent specificity for detection of Hepatocyte Growth Factor (HGF).
No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.
No significant cross-reactivity or interference between Hepatocyte Growth Factor (HGF) and analogues was observed.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle:
The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor (HGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor (HGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Growth Factor (HGF) level in the sample or standard.;
Uniprot ID:
C9E9X4