anti-LTbetaR (mouse) mAb (3C8)
| Code | Size | Price |
|---|
| AG-20B-0041-C100 | 100 ug | £270.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG1κ
Antibody Clonality: Monoclonal
Antibody Clone: 3C8
Regulatory Status: RUO
Target Species: Mouse
Application: Functional Study
Shipping:
-20°C
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
Lymphotoxin-beta Receptor; Tumor Necrosis Factor Receptor 2 Related Protein; Tumor Necrosis Factor C Receptor; Tumor Necrosis Factor Receptor Superfamily Member 3; TNFRSF3
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant mouse LTbetaR (cysteine-rich region/aa 31-221).
Long Description:
Monoclonal Antibody. Recognizes mouse LTbetaR. Isotype: Rat IgG1kappa. Clone: 3C8. Applications: FUNC (Blocking). Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. The LTbetaR activates two different NF-kappaB pathways that lead to distinct patterns of gene induction, including selected chemokines and the cytokine BAFF, which is essential for the survival of mature B lymphocytes. LTbetaR activates the classical NF-kappaB (relA/p50) pathway, like the type 1 TNF receptor (TNFR1), that regulates proinflammatory genes, like the chemokine MIP1beta. However, LTbetaR, unlike TNFR1, also activates the processing of p100 to form RelB/p52 complexes, which activate genes involved in lymphoid organ formation and lymphocyte survival.
NCBI, Uniprot Number:
P50284
Other data:
The monoclonal antibody to mouse LTbetaR is an agonist that can be used for the investigation of the regulation of BAFF (BlyS), chemokines and integrins using in vivo and tissue culture models, the development of NK cells and NK T cells, to study the regulation of NF-kappaB family of transcription factors in regulation of inflammation and homeostasis, particularly RelB NF-kappaB2 pathway. For use as an agonist the MAb to LTbetaR is added to cell cultures at 2µg/ml. For in vivo use, mice are injected intraperitoneally with 50µg of agonistic MAb to LTbetaR in sterile phosphate saline buffer.
Package Type:
Plastic Vial
Product Description:
The LTbetaR activates two different NF-kappaB pathways that lead to distinct patterns of gene induction, including selected chemokines and the cytokine BAFF, which is essential for the survival of mature B lymphocytes. LTbetaR activates the classical NF-kappaB (relA/p50) pathway, like the type 1 TNF receptor (TNFR1), that regulates proinflammatory genes, like the chemokine MIP1beta. However, LTbetaR, unlike TNFR1, also activates the processing of p100 to form RelB/p52 complexes, which activate genes involved in lymphoid organ formation and lymphocyte survival.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Recognizes mouse LTbetaR.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
A lymphotoxin-IFN-beta axis essential for lymphocyte survival revealed during cytomegalovirus infection: T.A. Banks, et al.; J. Immunol. 174, 7217 (2005) | Specific Remodeling of Splenic Architecture by Cytomegalovirus: C.A. Benedict et al.; PLoS Pathog. 2, e16 (2006) | LTbetaR Signaling Induces Cytokine Expression and Up-Regulates Lymphangiogenic Factors in Lymph Node Anlagen: M.F. Vondenhoff, et al.; J. Immunol. 182, 5439 (2009) | Phosphatase PTPN22 regulates dendritic cell homeostasis and cDC2 dependent T cell responses: H. A. Purvis, et al.; Front. Immunol. 11, 376 (2020)