anti-Giantin mAb (rec.) (TA10)

AdipoGen Life Sciences
Product Code: AG-27B-0003
Product Group: Primary Antibodies

CodeSizePrice
AG-27B-0003-C100100 ug£260.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Human
Antibody Isotype: IgG2λ
Antibody Clonality: Recombinant Antibody
Antibody Clone: TA10
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Immunocytochemistry (ICC)
Shipping:
-20°C
Storage:
-20°C

Images

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Structure of the recombinant antibody anti-Giantin (h), mAb (rec.) (TA10) (Prod. No. AG-27B-0003). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human Ig

Structure of the recombinant antibody anti-Giantin (h), mAb (rec.) (TA10) (Prod. No. AG-27B-0003). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human Ig

Further Information

Alternate Names/Synonyms:
Golgin Subfamily B Member 1; 372 kDa Golgi Complex-associated Protein; GCP372; Macrogolgin
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Human recombinant giantin.
Long Description:
Recombinant Antibody. Recognizes human and mouse giantin. Species cross-reactivity: Human, Mouse. Clone: TA10. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
NCBI, Uniprot Number:
Q14789
Other data:
anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Package Type:
Plastic Vial
Product Description:
Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
Purity:
>95% (SDS-PAGE)
Source / Host:
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Specificity:
Recognizes human and mouse giantin.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

References

Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003) | Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al; BMC Biotechnol. 10, 59 (2010)