ANGPTL4 (mouse) (rec.)

AdipoGen Life Sciences
Product Code: AG-40A-0075
Product Group: Recombinant Proteins

CodeSizePrice
AG-40A-0075-C01010 ug£150.00
Quantity:
AG-40A-0075-C05050 ug£290.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Monkey
Regulatory Status: RUO
Target Species: Mouse
Shipping:
Blue Ice
Storage:
-20°C

Images

1 / 1
ERK phosphorylation induced by hANGPTL4 in THP-1 cells.
THP-1 monocyte cells were serum starved for 16hr and then stimulated with ANGPTL4 (mouse) (rec.) (Prod. No. AG-40A-0075) (500ng/ml) for 1, 2 and 3 hrs, respectively. Antibodies against pERK1/2

ERK phosphorylation induced by hANGPTL4 in THP-1 cells.
THP-1 monocyte cells were serum starved for 16hr and then stimulated with ANGPTL4 (mouse) (rec.) (Prod. No. AG-40A-0075) (500ng/ml) for 1, 2 and 3 hrs, respectively. Antibodies against pERK1/2

Further Information

Alternate Names/Synonyms:
Angiopoietin-like Protein 4; FIAF; Fasting-induced Adipose Factor; HFARP; Hepatic Fibrinogen/Angiopoietin-related Protein
Biological Activity:
Activates ERK phosphorylation in THP-1 cells.
Concentration:
0.5mg/ml
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. 0.2µm-filtered solution in PBS.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Long Description:
Protein. Mouse ANGPTL4 (aa 1-410) is fused at the C-terminus to a FLAG®-tag. Source: COS-7 cells. Endotoxin content: <0.1EU/µg purified protein (LAL test; Lonza). Liquid. 0.2µm-filtered solution in PBS. Purity: >90% (SDS-PAGE). ANGPTL4 (Angiopoietin-like protein 4) mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorigenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.
Molecular Weight:
~50kDa (SDS-PAGE)
NCBI, Uniprot Number:
Q9Z1P8
Package Type:
Plastic Vial
Product Description:
ANGPTL4 (Angiopoietin-like protein 4) mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorigenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.
Purity:
>90% (SDS-PAGE)
Sequence:
Mouse ANGPTL4 (aa 1-410) is fused at the C-terminus to a FLAG®-tag.
Source / Host:
COS-7 cells
TAGs:
FLAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

References

Regulation of energy balance by the hypothalamic lipoprotein lipase regulator Angptl3: H.K. Kim, et al.; Diabetes 64, 1142 (2015)

Related Products

Product NameProduct CodeSupplier 
ANGPTL4 (fibrinogen-like domain) (human) (rec.)AG-40A-0070AdipoGen Life Sciences Summary Details