APRIL (human) (H98) (multimeric) (rec.)
| Code | Size | Price |
|---|
| AG-40B-0088-C010 | 10 ug | £290.00 |
Quantity:
| AG-40B-0088-3010 | 3 x 10 ug | £570.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species:
- Human
- Monkey
Shipping:
Blue Ice
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
MultimericAPRIL™; ACRP30headless:APRIL; ACRP30headless:CD256; ACRP30headless:TNFSF13; ACRP30headless:A-proliferation-inducing Ligand:
Biological Activity:
Stimulates B cell proliferation.
Concentration:
0.1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS + 0.5% Trehalose.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. The extracellular domain of human APRIL (aa 98-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG®-tag. Source: CHO cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS + 0.5% Trehalose. Binds to human and mouse BCMA and TACI. Does not bind to proteoglycans. Purity: >95% (SDS-PAGE). APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.
Molecular Weight:
~32kDa (SDS-PAGE)
NCBI, Uniprot Number:
O75888
Package Type:
Plastic Vial
Product Description:
APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.
Purity:
>95% (SDS-PAGE)
Sequence:
The extracellular domain of human APRIL (aa 98-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG®-tag.
Source / Host:
CHO cells
Specificity:
Binds to human and mouse BCMA and TACI. Does not bind to proteoglycans.
TAGs:
FLAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.