Netrin-1 (human) (rec.)
| Code | Size | Price |
|---|
| AG-40B-0040-C010 | 10 ug | £220.00 |
Quantity:
| AG-40B-0040-3010 | 3 x 10 ug | £430.00 |
Quantity:
| AG-40B-0040-C100 | 100 ug | £990.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species:
- Human
- Monkey
- Rat
Shipping:
Blue Ice
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
NTN1
Biological Activity:
Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.
Concentration:
10µg vial: 0.1mg/ml after reconstitution.100µg vial: 1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. Human netrin-1 (aa 25-604) is fused at the C-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity. Binds to human, mouse and rat UNC5B. Purity: >95% (SDS-PAGE). Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
Molecular Weight:
~80kDa (SDS-PAGE)
NCBI, Uniprot Number:
O95631
Package Type:
Plastic Vial
Product Description:
Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
Purity:
>95% (SDS-PAGE)
Sequence:
Human netrin-1 (aa 25-604) is fused at the C-terminus to a FLAG®-tag.
Source / Host:
HEK 293 cells
Specificity:
Binds to human, mouse and rat UNC5B.
TAGs:
FLAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
References
Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmadenci, et al.; Nat. Commun. 6, ID7398 (2015) | Targeting netrin?1/DCC interaction in diffuse large B?cell and mantle cell lymphomas: L. Broutier, et al.; EMBO Mol. Med. 8, 96 (2016) | Inhibition of DNA methylation promotes breast tumor sensitivity to netrin?1 interference: M. Grandin, et al.; EMBO Mol. Med. 8, 863 (2016) | Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers: M. Grandin, et al.; Cancer Cell 29, 173 (2016) | Ultrasound molecular imaging as a non-invasive companion diagnostic for netrin-1 interference therapy in breast cancer: J. Wischhusen, et al.; Theranostics 8, 5126 (2018)
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