LIF (human) (rec.)
| Code | Size | Price |
|---|
| AG-40B-0093-C010 | 10 ug | £120.00 |
Quantity:
| AG-40B-0093-3010 | 3 x 10 ug | £230.00 |
Quantity:
| AG-40B-0093-C100 | 100 ug | £760.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Shipping:
Blue Ice
Documents
Further Information
Alternate Names/Synonyms:
Leukemia Inhibitory Factor; Differentiation-stimulating Factor; D Factor; Melanoma-derived LPL Inhibitor
Biological Activity:
Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.
Concentration:
AG-40B-0093-C010/AG-40B-0093-3010: 0.1mg/ml after reconstitution.AG-40B-0093-C100: 1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.05EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. Human LIF (aa 23-202) is fused at the N-terminus to a tag. Source: E. coli. Endotoxin content: <0.05EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Mouse and human embryonic stem cells. Purity: >95% (SDS-PAGE). LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Molecular Weight:
~46kDa (SDS-PAGE)
NCBI, Uniprot Number:
P15018
Package Type:
Plastic Vial
Product Description:
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Purity:
>95% (SDS-PAGE)
Sequence:
Human LIF (aa 23-202) is fused at the N-terminus to a tag.
Source / Host:
E. coli
Specificity:
Mouse and human embryonic stem cells.
TAGs:
GST
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.