IL-27 (mouse):Fc (human) (rec.)

Chimerigen
Product Code: CHI-MF-11127
Product Group: Recombinant Proteins
Supplier: Chimerigen

CodeSizePrice
CHI-MF-11127-C05050 ug£425.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Hamster
Regulatory Status: RUO
Target Species: Mouse
Shipping:
BI
Storage:
-20°C

Further Information

Alternate Names/Synonyms:
Interleukin-27
Biological Activity:
Bioactivity was measured in a cell proliferation assay of Con A activated mouse splenocytes.
EClass:
32160000
Endotoxin:
<0.06EU/µg protein (LAL test; Lonza).
Form (Short):
solid
Formulation:
Lyophilized from 0.2µm-filtered solution in PBS.
Handling Advice:
Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Protein. The mouse IL-27 complex composed of the Ebi3 subunit (aa 1-228) and the p28 subunit (aa 29-234) is fused through a polypeptide linker to the Fc region of human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/µg protein (LAL test; Lonza). Lyophilized from 0.2µm-filtered solution in PBS. Purity: >98% (SDS-PAGE). Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an antiinflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on na?e CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction.
NCBI, Uniprot Number:
AAI19403.1
Package Type:
Plastic Vial
Product Description:
Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an antiinflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on na?e CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction.
Purity:
>98% (SDS-PAGE)
Sequence:
The mouse IL-27 complex composed of the Ebi3 subunit (aa 1-228) and the p28 subunit (aa 29-234) is fused through a polypeptide linker to the Fc region of human IgG1.
Source / Host:
CHO cells
TAGs:
Fc
Transportation:
Non-hazardous
UNSPSC Category:
Interleukins
UNSPSC Number:
41116127
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.