Caspase-1 (mouse) Matched Pair Detection Set
| Code | Size | Price |
|---|
| AG-46B-0003-KI01 | 1 Set | £570.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Blue Ice
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
Interleukin-1beta Convertase; IL-1BC; Interleukin-1beta-converting Enzyme; ICE
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Handling Advice:
Avoid freeze/thaw cycles.
Kit Contains:
1 vial standard protein (1µg) (lyophilized) [STD] | 1 vial coating antibody (120µg) [COAT] | 1 vial detection antibody (60µg) [DET] | 1 vial streptavidin-HRP (10µg) (lyophilized) [STREP]
Long Description:
Matched Pair Detection Set. Detects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1. Colorimetric assay. Sample Type: Cell Culture Supernatant. Range: 0.15ng/ml to 10ng/ml. Sensitivity: 50pg/ml. Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
NCBI, Uniprot Number:
P29452
Package Type:
Box
Product Description:
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
Range:
0.15ng/ml to 10ng/ml.
Sample Type:
Cell Culture Supernatant
Sensitivity:
50pg/ml
Specificity:
Detects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1.
Transportation:
Non-hazardous
UNSPSC Category:
ELISA Kits
UNSPSC Number:
41116126
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
ATG16L1 deficiency in macrophages drives clearance of uropathogenic E. coli in an IL-1b-dependent manner: J.W. Symington, et al.; Muc. Immunol. 8, 1388 (2015)