anti-Caspase-1 (p20) (human) mAb (Bally-1) (Biotin)
| Code | Size | Price |
|---|
| AG-20B-0048B-C100 | 100 ug | £400.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Shipping:
Blue Ice
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Interleukin-1 beta Convertase; IL-1BC; Interleukin-1 beta-converting Enzyme; ICE
Concentration:
0.5mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant human caspase-1.
Labels - Conjugates:
Biotin
Long Description:
Monoclonal Antibody. Recognizes endogenous full-length and activated (p20 fragment) human caspase-1. Isotype: Mouse IgG1. Clone: Bally-1. Applications: WB. Liquid. In PBS containing 0.02% sodium azide. Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
NCBI, Uniprot Number:
P29466
Package Type:
Plastic Vial
Product Description:
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Recognizes endogenous full-length and activated (p20 fragment) human caspase-1.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibody Conjugates
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012) | Liver X receptor beta activation induces pyroptosis of human and murine colon cancer cells: V. Derangere, et al.; Cell Death Differ. 21, 1914 (2014) | Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling: N. Kayagaki, et al.; Nature 526, 666 (2015) | Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016) | Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016) | The TAT-RasGAP317-326 anti-cancer peptide can kill in a caspase-, apoptosis-, and necroptosis-independent manner: M. Heulot, et al.; Oncotarget 7, 6342 (2016) | Impact of human monocyte and macrophage polarization on NLR expression and NLRP3 inflammasome activation: F. Awad, et al.; PLoS ONE 12, e0175336 (2017) | The DNA inflammasome in human myeloid cells is initiated by a STING-cell death program upstream of NLRP3: M.M. Gaidt, et al.; Cell 171, 1110 (2017) | Calcium phosphate particles stimulate interleukin-1beta release from human vascular smooth muscle cells: A role for spleen tyrosine kinase and exosome release: Y. Dautova, et al.; J. Mol. Cell Cardiol. 115, 82 (2017) | POH1 deubiquitinates pro-interleukin-1beta and restricts inflammasome activity: L. Zhang, et al.; Nat. Commun. 9, 4225 (2018) | SUMO-mediated regulation of NLRP3 modulates inflammasome activity: R. Barry, et al.; Nat. Commun. 9, 3001 (2018) | The Crohn?s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019) | Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis: D. Fisch, et al.; EMBO J. 38, e100926 (2019) | HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Diff. 10, 256 (2019) | Inflammasome Activation Triggers Blood Clotting and Host Death through Pyroptosis: C. Wu, et al.; Immunity 50, 1401 (2019) | SERPINB1-mediated checkpoint of inflammatory caspase activation: Y.J. Choi, et al.; Nat. Immunol. 20, 276 (2019) [Knock Down Validation] | NLRP3 inflammasome activation drives tau pathology: C. Ising, et al.; Nature 575, 669 (2019) | Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated Death: D. Fisch, et al.; Cell Rep. 32, 108008 (2020) | Molecularly Distinct NLRP3 Inducers Mediate Diverse Ratios of Interleukin-1beta and Interleukin-18 from Human Monocytes: K. Midtbo, et al.; Med. Inflamm. ID4651090 (2020) | Cross-regulation between LUBAC and caspase-1 modulates cell death and inflammation: T. Douglas & M. Saleh; J. Biol. Chem. 295, 5216 (2020) | AIM2 Inhibits BRAF-Mutant Colorectal Cancer Growth in a Caspase-1-Dependent Manner: S. Shah, et al.; Front. Cell Dev. Biol. 9, 588278 (2021) | Human NLRP1 is a sensor for double-stranded RNA: S. Bauernfried, et al.; Science 371, 482 (2021)