Caspase-1 (mouse) ELISA Kit
| Code | Size | Price |
|---|
| AG-45B-0002-KI01 | 96 wells | £540.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Shipping:
Blue Ice
Storage:
+4°C
Further Information
Alternate Names/Synonyms:
Interleukin-1beta Convertase; IL-1BC; Interleukin-1beta-converting Enzyme; ICE
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Handling Advice:
After standard reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Plate and reagents should reach room temperature before use.
Long Description:
ELISA Assay. Detects mouse caspase-1 (proform as well as p10 and p20 domain). Does not detect human caspase-1. Colorimetric assay. Sample Types: Cell Culture Supernatant, Plasma, Serum. Range: 0.15ng/ml to 10ng/ml. Sensitivity: 33pg/ml. Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. This Caspase-1 (mouse) ELISA Kit can be used to measure caspase-1 (mouse) in cell culture supernatants, serum and plasma. It is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
NCBI, Uniprot Number:
P29452
Package Type:
Box
Product Description:
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. This Caspase-1 (mouse) ELISA Kit can be used to measure caspase-1 (mouse) in cell culture supernatants, serum and plasma. It is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
Range:
0.15ng/ml to 10ng/ml
Sample Type:
Cell Culture Supernatant, Plasma, Serum
Sensitivity:
33pg/ml
Specificity:
Detects mouse caspase-1 (proform as well as p10 and p20 domain). Does not detect human caspase-1.
Transportation:
Non-hazardous
UNSPSC Category:
ELISA Kits
UNSPSC Number:
41116126
Use & Stability:
12 months after the day of manufacturing. See expiry date on ELISA Kit box.
References
Pharmacologic inhibition of IkappaB kinase activates immediate hypersensitivity reactions in mice: D. Miyazaki, et al.; Am. J. Pathol. 183, 96 (2013) | Alendronate augments lipid A-induced IL-1beta release and Smad3/NLRP3/ASC-dependent cell death: R. Tamai & Y. Kiyoura; Life Sci. 198, 8 (2018) | Inflammatory Monocytes Drive Influenza A Virus-Mediated Lung Injury in Juvenile Mice: B.M. Coates, et al.; J. Immunol. 200, 2391 (2018) | Leishmania Lipophosphoglycan Triggers Caspase-11 and the Non-canonical Activation of the NLRP3 Inflammasome: R.V.H. de Carvalho, et al.; Cell Rep. 26, 429 (2019)