IL-1alpha (mouse) Matched Pair Detection Set
| Code | Size | Price |
|---|
| AG-46B-0004-KI01 | 1 Set | £570.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Blue Ice
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
Interleukin-1alpha
Assay Type:
Sandwich
Detection Type:
Colorimetric
EClass:
32160000
Handling Advice:
Avoid freeze/thaw cycles.
Kit Contains:
1 vial standard protein (1µg) (lyophilized) [STD] | 1 vial coating antibody (120µg) [COAT] | 1 vial detection antibody (30µg) [DET] | 1 vial streptavidin-HRP (10µg) (lyophilized) [STREP]
Long Description:
Matched Pair Detection Set. Detects mouse IL-1alpha. Does not detect human IL-1alpha or mouse IL-1beta. Colorimetric assay. Sample Type: Cell Culture Supernatant. Range: 15.6 to 2000pg/ml. Sensitivity: 10pg/ml. The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.
NCBI, Uniprot Number:
P01582
Package Type:
Box
Product Description:
The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.
Range:
15.6 to 2000pg/ml
Sample Type:
Cell Culture Supernatant
Sensitivity:
10pg/ml
Specificity:
Detects mouse IL-1alpha. Does not detect human IL-1alpha or mouse IL-1beta.
Transportation:
Non-hazardous
UNSPSC Category:
ELISA Kits
UNSPSC Number:
41116126
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.