anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (preservative free)

AdipoGen Life Sciences
Product Code: AG-20B-0043PF
Product Group: Primary Antibodies

CodeSizePrice
AG-20B-0043PF-C500500 ug£250.00
Quantity:
AG-20B-0043PF-M0022 mg£470.00
Quantity:
AG-20B-0043PF-M01010 mg£1,410.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Isotype: Rat IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: Nimp-R14
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
Shipping:
-20°C

Images

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Mouse neutrophils are depleted in vivo by anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (preservative free) (AG-20B-0043PF).  Method: Mice were injected) i.p. with 250?g of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (pre

Mouse neutrophils are depleted in vivo by anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (preservative free) (AG-20B-0043PF).  Method: Mice were injected) i.p. with 250?g of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (pre

Further Information

Concentration:
Lot dependent
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In PBS.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Purified mouse BALB/c neutrophils.
Labels - Conjugates:
Preservative Free
Long Description:
Monoclonal Antibody. Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes). Isotype: Rat IgG2b. Clone: Nimp-R14. Applications: FACS, FUNC (Blocking), ICC, IHC. Liquid. In PBS containing 10% glycerol. Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.
NCBI, Uniprot Number:
P35461
Other data:
10mg Size: Delivered as 5 x 2mg vials. Negative control: AG-35B-0011 http://www.adipogen.com/ag-35b-0011/rat-igg2b-isotype-control.html
Package Type:
Plastic Vial
Product Description:
Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb allows the depletion of mouse neutrophils and monocytes in vivo.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes).
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies: A. Lopez, et al.; Brit. J. Haematol. 57, 489 (1984) | Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells: D. McLaren, et al.; Parasite Immunol. 9, 667 (1987) | Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice: P. Van Lent, et al.; J. Leukoc. Biol. 61, 267 (1997) | Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction: E. Lubberts, et al.; J. Immunol. 163, 4546 (1999) | An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major: F. Tacchini-Cottier, et al.; J. Immunol. 165, 628 (2000) | A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5: K.M. Al-Qaoud, et al.; Int. Immunol. 12, 899 (2000) | Rapid recruitment of inflammatory monocytes is independent of neutrophil migration: R.B. Henderson, et al.; Blood 102, 328 (2003) | Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils: B. de Vries, et al.; J. Immunol. 170, 3883 (2003) | Absence of cross-reactivity between murine Ly-6C and Ly-6G: S. Nagendra, et al.; Cytom. A 58, 195 (2004) | Activation of Toll-Like Receptor (TLR)2, TLR4, and TLR9 in the Mammalian Cornea Induces MyD88-Dependent Corneal Inflammation: A.C. Johnson, et al.; Invest. Ophthalmol. Vis. Sci. 46, 589 (2005) | Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani: E. McFarlane, et al.; Infect. Immun. 76, 532 (2008) | Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections: A. Navarini, et al.; PNAS 106, 7107 (2009) | Analysis of innate defences against Plasmodium falciparum in immunodeficient mice: A Ludovic, et al.; Malaria J. 9, 197 (2010) | Role of Neutrophils in the Early Shaping of the Leishmania Major Specific Immune Response in Experimental Murine Cutaneous Leishmaniasis: M. Charmoy, et al.; Neutrophils in Infectious Diseases 1, 49 (2011)