(R)-FSL-1
| Code | Size | Price |
|---|
| AG-CP3-0010-C100 | 100 ug | £220.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
Fibroblast-stimulating Lipopeptide; R-Pam2CGDPKHPKSF; (R)-Pam2Cys-Gly-Asp-Pro-Lys-His-Pro-Lys-Ser-Phe-OH
Appearance:
Colorless powder.
CAS:
929905-36-2
EClass:
32160000
Form (Short):
solid
Formulation:
Lyophilized.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
InChi:
InChI=1S/C84H140N14O18S/c1-3-5-7-9-11-13-15-17-19-21-23-25-30-44-74(103)115-56-62(116-75(104)45-31-26-24-22-20-18-16-14-12-10-8-6-4-2)57-117-58-63(87)76(105)89-54-72(100)91-67(52-73(101)102)83(112)98-49-37-43-71(98)81(110)92-64(40-32-34-46-85)77(106)94-66(51-61-53-88-59-90-61)82(111)97-48-36-42-70(97)80(109)93-65(41-33-35-47-86)78(107)96-69(55-99)79(108)95-68(84(113)114)50-60-38-28-27-29-39-60/h27-29,38-39,53,59,62-71,99H,3-26,30-37,40-52,54-58,85-87H2,1-2H3,(H,88,90)(H,89,105)(H,91,100)(H,92,110)(H,93,109)(H,94,106)(H,95,108)(H,96,107)(H,101,102)(H,113,114)/t62-,63+,64+,65+,66+,67+,68+,69+,70+,71+/m1/s1
InChiKey:
VJJLLNHELGKSNA-QWIIDARVSA-N
Long Description:
Chemical. Formula: C84H140N14O18S. MW: 1666.2. Synthetic. R-FSL-1 (R-Pam2CGDPKHPKSF) is a synthetic lipoprotein that represents the N-terminal part of the 44kDa lipoprotein LP44 of Mycoplasma salivarium. The naturally occurring R-stereoisomer is biologically more active than the S-stereoisomer. Stimulator of TLR2/TLR6. Inducer of TNF-alpha production in macrop
MDL:
N/A
Molecular Formula:
C84H140N14O18S
Molecular Weight:
1666.2
Other data:
Note: FSL-1 stimulation induces a MyD88-dependent signaling cascade leading to the activation of NF-kappaB and the production of proinflammatory cytokines. FSL-1 exhibits a higher stimulatory activity than MALP-2 when tested for its ability to induce the production of TNF-alpha in macrophages and NF-kappaB in TLR2/TLR6 transfected HEK293 cells. Depending on the sensitivity of the in vitro assay, the recommended working concentration for specific stimulation of innate immunity via TLR2/TLR6 heterodimers is 10-100nM. FSL-1 is water soluble in contrast to MALP2.
Package Type:
Vial
Product Description:
R-FSL-1 (R-Pam2CGDPKHPKSF) is a synthetic lipoprotein that represents the N-terminal part of the 44kDa lipoprotein LP44 of Mycoplasma salivarium [1]. The naturally occurring R-stereoisomer is biologically more active than the S-stereoisomer [4]. Stimulator of TLR2/TLR6 [2-13]. Inducer of TNF-alpha production in macrophages [5]. Upregulates proinflammatory cytokines [3,5]. Activator of the proinflammatory transcription factor NF-kappaB [3].
Purity:
Absence of detectable protein or DNA contaminants with agonistic TLR activity.
SMILES:
[H][C@@](COC(=O)CCCCCCCCCCCCCCC)(CSC[C@]([H])(N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=CN1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O)OC(=O)CCCCCCCCCCCCCCC
Solubility Chemicals:
Soluble in water (10mg/ml). Use a homogeniser or sonicator to prepare a homogenous solution.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
The N-terminal lipopeptide of a 44-kDa membrane-bound lipoprotein of Mycoplasma salivarium is responsible for the expression of intercellular adhesion molecule-1 on the cell surface of normal human gingival fibroblasts: K.I. Shibata, et al.; J. Immunol. 165, 6538 (2000) | Discrimination of bacterial lipoproteins by Toll-like receptor 6: O. Takeuchi, et al.; Int. Immunol. 13, 933 (2001) | Relationship between structures and biological activities of mycoplasmal diacylated lipopeptides and their recognition by Toll-Like Receptors 2 and 6: T. Okusawa, et al.; Infect. Immun. 72, 1657 (2004) | Involvement of suppressors of cytokine signaling in toll-like receptor-mediated block of dendritic cell differentiation: H. Bartz, et al.; Blood 108, 4102 (2006) | Effects of repeated injections of fibroblast-stimulating lipopeptide-1 on fever, formation of cytokines, and on the responsiveness to endotoxin in guinea-pigs: A. Greis, et al.; Acta Physiol. 197, 35 (2009) | Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide: S. Riekenberg, et al.; FEBS J. 276, 649 (2009) | Toll-like receptors and innate immunity: H. Kumar, et al.; BBRC 388, 621 (2009) | Lipoteichoic Acid Induces Unique Inflammatory Responses when Compared to Other Toll-Like Receptor 2 Ligands: E.M. Long, et al.; PLoS One 4, e5601 (2009) | A new cell-based innate immune receptor assay for the examination of receptor activity, ligand specificity, signalling pathways and the detection of pyrogens: A. Burger-Kentischer, et al.; J. Immunol. Methods 358, 93 (2010) | Toll-like receptor 2 (P631H) mutant impairs membrane internalization and is a dominant negative allele: G.E. Etokebe, et al.; Scand. J. Immunol. 71, 369 (2010) | TLR2 stimulation drives human naive and effector regulatory T cells into a Th17-like phenotype with reduced suppressive function: M.H. Nyirenda, et al.; J. Immunol. 187, 2278 (2011) | A subclass of acylated anti-inflammatory mediators usurp Toll-like receptor 2 to inhibit neutrophil recruitment through peroxisome proliferator-activated receptor gamma: E.M. Long, et al.; PNAS 108, 16357 (2011) | Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response: K. Wilhelmsen, et al.; Innate Immun. 18, 602 (2012) | FcepsilonR1-mediated mast cell reactivity is amplified through prolonged Toll-like receptor-ligand treatment: R. Saluja, et al.; PLoS One 7, e43547 (2012)