14-3-3 sigma Antibody

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ProSci
Product Code: PSI-45-174
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-174-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-174 (0.03ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-174 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-174 (0.03ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-174 (0.03ug/ml) staining of Mouse (A) and Rat (B) Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26-28kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4ug/ml.
Background References:
  • Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr;15(4):237-46. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-DLHTLSEDSYKDST
NCBI Gene ID #:
2810
NCBI Official Name:
stratifin
NCBI Official Symbol:
SFN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006133.1
Protein GI Number:
5454052
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P31947