14-3-3 Antibody

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ProSci
Product Code: PSI-50-231
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-231-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Canine (dog)
  • Chicken (Gallus)
  • Frog
  • Human
  • Mouse
  • Rat
  • Sheep
  • Zebrafish
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
14-3-3 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat brainstem lysate showing specific immunolabeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).
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Western blot of rat brainstem lysate showing specific immunolabeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).

Documents

Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots with human and rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen for this antibody.
Background:
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).
Background References:
  • Berg D, Holzmann C, Riess O (2003) 14-3-3 Proteins in the nervous system. Nat Rev Neurosci 4:752-762.
  • Bridges D, Moorhead GB (2005) 14-3-3 Proteins: a number of functions for a numbered protein. Sci STKE 2005:re10.
  • Dougherty MK, Morrison DK (2004) Unlocking the code of 14-3-3. J Cell Sci 117:1875-1884.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat 14-3-3 protein.
Modifications:
Phospho-Specific
NCBI Gene ID #:
56011
NCBI Official Name:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
NCBI Official Symbol:
Ywhab
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29
Protein Accession #:
P35213
Protein GI Number:
1345591
Purification:
Affinity Purified
Research Area:
Phospho-Specific
Swissprot #:
P35213
User NOte:
Optimal dilutions for each application to be determined by the researcher.