COMP (rat):Angiopoietin-1 (human) (rec.)

AdipoGen Life Sciences
Product Code: AG-40B-0147
Product Group: Recombinant Proteins

CodeSizePrice
AG-40B-0147-C01010 ug£200.00
Quantity:
AG-40B-0147-C05050 ug£630.00
Quantity:
AG-40B-0147-50105 x 10 ug£685.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
-20°C

Images

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COMP (rat):Angiopoietin-1 (human) (rec.) (AG-40B-0147) binds to Tie-2 (human):Fc (human). Method: Tie-2 (human):Fc was coated on an ELISA plate at 1?g/ml. After blocking and washing steps, indicated concentrations of COMP (rat):Angiopoietin-1 (huma

COMP (rat):Angiopoietin-1 (human) (rec.) (AG-40B-0147) binds to Tie-2 (human):Fc (human). Method: Tie-2 (human):Fc was coated on an ELISA plate at 1?g/ml. After blocking and washing steps, indicated concentrations of COMP (rat):Angiopoietin-1 (huma

Further Information

Alternate Names/Synonyms:
COMP-Ang1; COMP:Ang-1; COMP Angiopoietin 1; COMP ANGPT1
Biological Activity:
More potent than native angiopoietin-1. The protein forms pentameric structures.
Concentration:
After reconstitution:for 10µg size: 0.1mg/mlfor 50µg size: 1 mg/ml
EClass:
32160000
Endotoxin:
<0.05EU/µg purified protein (LAL test).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS + 0.02% CHAPS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. The fibrinogen-like domain (aa 284-498) of human angiopoietin-1 is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag. Source: CHO cells. Endotoxin content: <0.05EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS + 0.02% CHAPS. Binds to human angiopoietin-1 receptor (Tie-2). Purity: >95% (SDS-PAGE). Soluble, stable and more potent angiopoietin-1 (Ang-1) variant.
Molecular Weight:
35kDa (SDS-PAGE)
NCBI, Uniprot Number:
Q15389 (Ang-1, human
Package Type:
Plastic Vial
Product Description:
Soluble, stable and more potent angiopoietin-1 (Ang-1) variant.
Purity:
>95% (SDS-PAGE)
Sequence:
The fibrinogen-like domain (aa 284-498) of human angiopoietin-1 is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag.
Source / Host:
CHO cells
Specificity:
Binds to human angiopoietin-1 receptor (Tie-2).
TAGs:
FLAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

References

COMP-Ang1: a designed angiopoietin-1 variant with nonleaky angiogenic activity: CH. Cho, et al.; PNAS 101, 5547 (2004) | COMP-angiopoietin-1 promotes wound healing through enhanced angiogenesis, lymphangiogenesis, and blood flow in a diabetic mouse model: CH. Cho, et al.; PNAS 103, 4946 (2006) | Protective role of COMP-Ang1 in ischemic rat brain: HY. Shin, et al.; J. Neurosci. Res. 88, 1052 (2010) | COMP-angiopoietin-1 promotes cavernous angiogenesis in a type 2 diabetic rat model: SO. Kim, et al.; J. Korean Med. Sci. 28, 725 (2013) | COMP-Ang1 accelerates chondrocyte maturation by decreasing HO-1 expression: S. Kim, et al.; J. Cell. Biochem. 114, 2513 (2013) | EphrinB2/EphB4 signaling regulates non?sprouting angiogenesis by VEGF: E. Groppa, et al.; EMBO Rep. e45054 (2018)