anti-Apaf-1 (mouse/rat) mAb (13F11)

AdipoGen Life Sciences
Product Code: AG-20T-0133
Product Group: Primary Antibodies

CodeSizePrice
AG-20T-0133-C100100 ug£270.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
-20°C
Storage:
-20°C

Images

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Western blot analysis using anti-Apaf-1 (mouse/rat), MAb (13F11) (AG-20T-0133) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.

Western blot analysis using anti-Apaf-1 (mouse/rat), MAb (13F11) (AG-20T-0133) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.

Further Information

Alternate Names/Synonyms:
Apoptosis Protease-activating Factor 1
Concentration:
1mg/ml
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
Long Description:
Monoclonal Antibody. Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1. Isotype: Rat IgG2akappa. Clone: 13F11. Applications: ELISA, ICC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
NCBI, Uniprot Number:
Q9EPV5
Package Type:
Plastic Vial
Product Description:
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
Purity:
>95% (SDS-PAGE)
Specificity:
Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

Histone deacetylase activity regulates apaf-1 and caspase 3 expression in the developing mouse retina: D.M. Wallace, et al.; Invest. Ophthalmol. Vis. Sci. 47, 2765 (2006) | Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues: C.E. Johnson, et al.; PNAS 104, 20820 (2007) | Chromatin modi?cation of Apaf-1 restricts the apoptotic pathway in mature neurons: K.M. Wright, et al.; J. Cell Biol. 179, 825 (2007) | Inhibition of apoptosome formation by suppression of Hsp90beta phosphorylation in tyrosine kinase-induced leukemias: M. Kurokawa, et al.; Mol. Cell Biol. 28, 5494 (2008) | Suppression of the intrinsic apoptosis pathway by synaptic activity: F. Leveille, et al.; J. Neurosci. 30, 2623 (2010)