anti-Caspase-2 mAb (11B4)

AdipoGen Life Sciences
Product Code: AG-20T-0136
Product Group: Primary Antibodies

CodeSizePrice
AG-20T-0136-C100100 ug£300.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Multiple species
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
-20°C
Storage:
-20°C

Images

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Western blot analysis using anti-caspase-2, mAb (11B4) (Prod. No. AG-20T-0136), detecting caspase-2 expression in cultured mouse and human cell lines. Caspase-2 protein was revealed in lysates from 2x105 mouse and human cell lines by 11B4 and E

Western blot analysis using anti-caspase-2, mAb (11B4) (Prod. No. AG-20T-0136), detecting caspase-2 expression in cultured mouse and human cell lines. Caspase-2 protein was revealed in lysates from 2x105 mouse and human cell lines by 11B4 and E

Further Information

Alternate Names/Synonyms:
CASP-2; Protease ICH-1; Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2; NEDD-2
Concentration:
1mg/ml
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant human caspase-2 (p19 subunit).
Long Description:
Monoclonal Antibody. Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot. Isotype: Rat IgG2akappa. Clone: 11B4. Applications: ELISA, FACS, ICC, IHC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.
NCBI, Uniprot Number:
P42575
Package Type:
Plastic Vial
Product Description:
Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.
Purity:
>95% (SDS-PAGE)
Specificity:
Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002) | A novel Apaf-1?independent putative caspase-2 activation complex: S.H. Read, et al.; J. Cell Biol. 159, 739 (2002) | A Novel Caspase-2 Complex Containing TRAF2 and RIP1: M. Lamkanfi, et al.; J. Biol. Chem. 280, 6923 (2005) | Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex: H.H. Park, et al.; Cell 128, 533 (2007) | Caspase-2 activation in the absence of PIDDosome formation: C. Manzl, et al.; J. Cell Biol. 185, 291 (2009) | LBH589, a deacetylase inhibitor, induces apoptosis in adult T-cell leukemia/lymphoma cells via activation of a novel RAIDD-caspase-2 pathway: H. Hasegawa, et al.; Leukemia 25, 575 (2011)