anti-Caspase-12 (mouse) mAb (12G6)

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AdipoGen Life Sciences
Product Code: AG-20T-0141
Product Group: Primary Antibodies
CodeSizePrice
AG-20T-0141-C100100 ug£250.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Western Blot (WB)
Shipping:
-20°C
Storage:
-20°C

Images

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Western blot analysis using anti-caspase-12 (mouse), mAb (12G6) (Prod. No. AG-20T-0141) detecting, A) endogenous caspase-12 in mouse lung, liver, spleen and pancreas, and B) pro-caspase-12 in mouse MEF?s.
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Western blot analysis using anti-caspase-12 (mouse), mAb (12G6) (Prod. No. AG-20T-0141) detecting, A) endogenous caspase-12 in mouse lung, liver, spleen and pancreas, and B) pro-caspase-12 in mouse MEF?s.

Documents

Further Information

Alternate Names/Synonyms:
CASP-12
Concentration:
1mg/ml
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa 183-205 of mouse caspase-12.
Long Description:
Monoclonal Antibody. Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11. Isotype: Rat IgG1kappa. Clone: 12G6. Applications: ELISA, FACS, WB. Liquid. In PBS containing 0.02% sodium azide. Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.
NCBI, Uniprot Number:
O08736
Package Type:
Plastic Vial
Product Description:
Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.
Purity:
>95% (SDS-PAGE)
Specificity:
Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.