sFas Ligand Recombinant Protein

ProSci
Product Code: PSI-40-531
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-40-531-0.002mg0.002mg£300.00
Quantity:
PSI-40-531-0.01mg0.01mg£428.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized sFas Ligand recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted sFas Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any pr

Documents

Further Information

Additional Names:
APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL
Background:
Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.
Biological Activity:
Biological activity was determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤ 10.0 ng/mL, corresponding to a specific activity of ≥ 1 x 105 units/mg.
NCBI Gene ID #:
356
NCBI Official Name:
Fas ligand (TNF superfamily, member 6)
NCBI Official Symbol:
FASLG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_000630.1
Protein GI Number:
4557329
Purity:
Greater than or equal to 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
CHO cells
Swissprot #:
P48023