Wnt-1 Recombinant Protein

ProSci
Product Code: PSI-40-216
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-40-216-0.002mg0.002mg£300.00
Quantity:
PSI-40-216-0.01mg0.01mg£428.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Storage:
The lyophilized Wnt-1 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Wnt-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, expos

Documents

Further Information

Additional Names:
INT1, OI15, BMND16, INT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog
Background:
Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
Biological Activity:
The ED50 was determined by its ability to enhance BMP - 2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/mL in the presence of 200 ng/mL of human BMP - 2.
NCBI Gene ID #:
7471
NCBI Official Name:
wingless-type MMTV integration site family, member 1
NCBI Official Symbol:
WNT1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_005421.1
Protein GI Number:
4885655
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Recombinant Protein Sequence:
#N/A
Source:
E. coli
Swissprot #:
P04628