IRAK Recombinant Protein
| Code | Size | Price |
|---|
| PSI-95-112-0.05mg | 0.05mg | £347.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Mass Spectrometry
- Neutralisation
- Western Blot (WB)
Storage:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Documents
Further Information
Additional Names:
IRAK Antibody: IRAK, pelle, IRAK, Interleukin-1 receptor-associated kinase 1, IRAK-1
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Background:
Nuclear factor kappa B (NF-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1,2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-B activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1,4).
Background References:
- Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
- Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.
- Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.
- Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.
Buffer:
100mM sodium phosphate, 500mM NaCl, 25 mM imidazole, 10% gycerol
Fusion Tag:
Fusion Partner: C-terminal His-tag
NCBI Gene ID #:
3654
NCBI Official Name:
interleukin-1 receptor-associated kinase 1
NCBI Official Symbol:
IRAK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
15 kDa (Calculated)
Protein Accession #:
P51617
Protein GI Number:
8928535
Purity:
~95%
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P51617