IgG2 Fc Region Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-413-0.2mg | 0.2mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).
Background References:
- Sikorav J.-L., et al., 1980, Nucleic Acids Res. 8:3143-3155.
- Ollo R., et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:2442-2446.
Biological Activity:
Measured by its ability to bind rhCD32a in a functional ELISA.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
NCBI Gene ID #:
3501
NCBI Official Name:
immunoglobulin heavy constant gamma 2
NCBI Official Symbol:
IGHG2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
25.7 kDa
Protein Accession #:
P01859
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P01859