CD36 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-121-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CD36, SCARB3, BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Platelet Glycoprotein 4, glycoprotein IV, gpIV, glycoprotein IIIb, gpIIIb
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
CD36 (Cluster of Differentiation 36) is also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 In the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macropinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.
Background References:
- Husemann, J. et al., 2002, Glia 40:195.
- Armstrong, L and P. Bornstein, 2003, Matrix. Biol. 22:63.
- Collins RF, et al., 2009, J. Biol. Chem. 284 (44): 30288?97.
- Stewart CR, et al., 2010, Nat. Immunol. 11 (2): 155?61.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized rhCD36 at 2μg/mL (100μL/well) can bind rhTSP2/His with a linear range of 0. 01-1μg/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
948
NCBI Official Name:
CD36 molecule (thrombospondin receptor)
NCBI Official Symbol:
CD36
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
47.5 kDa
Protein Accession #:
NP_001001547.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P16671