CRP Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-203-0.25mg | 0.25mg | £452.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CRP, C-reactive Protein
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively. CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.
Background References:
- Pepys MB, Hirschfield GM. 2003, J Clin Invest 111 (12): 1805?12.
- Lau DC., 2005, Am J Physiol Heart Circ Physiol 288 (5): H2031?41.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CRP/ C-reactive Protein at 5μg/mL, can bind Biotinylated Human FcGR2A /CD32a Protein with a linear range of 0. 1-10μg/mL. ??
Buffer:
200 mM Tris, 1M NaCl, pH8.0
Fusion Tag:
His Tag
NCBI Gene ID #:
1401
NCBI Official Name:
C-reactive protein, pentraxin-related
NCBI Official Symbol:
CRP
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
24 kDa
Protein Accession #:
NP_000558.2
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P02741