TNFR1 Recombinant Protein

ProSci
Product Code: PSI-96-727
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-96-727-0.1mg0.1mg£597.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Documents

Further Information

Additional Names:
TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Tumor necrosis factor receptor 1 (TNF-R1) is also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, which belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF).
Background References:
  • Jiang Y., et al., 1999, Science 283:543-546.
  • Le V.T., et al., 2011, J. Virol. 85:13260-13270.
  • Gregory A.P., et al., 2012, Nature 488:508-511.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized human TNFa at 5μg/mL (100μl/well) can bind human TNFR1 / CD120a. The EC50 of human TNFR1 is 510 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
7132
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 1A
NCBI Official Symbol:
TNFRSF1A
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
22.3 kDa
Protein Accession #:
NP_001056
Purity:
>92% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P19438