TNFRII Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-731-0.05mg | 0.05mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
TNF RI is also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with highaffinity.Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage?pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.
Background References:
- Alicia SC, et al., 2005, Molecular and Cellular Biology, 25, No. 11: 4716-26.
- Ziegenhagen, MW., et al., 2000, Journal of Internal Medicine, 248(1): 33-41.
- Iris Shai, et al., 2005, Diabetes Care, Vol. 28, No. 6: 1376-1382.
- MacEwan, D.J., 2002, Cell Signal. 14:477.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized human TNFa at 5μg/mL (100μl/well) can bind human TNFR2. The EC50 of human TNFR2 is 647 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
7133
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 1B
NCBI Official Symbol:
TNFRSF1B
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
26 kDa
Protein Accession #:
AAA36755
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P20333