Sclerostin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-397-0.1mg | 0.1mg | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
SOST, VBCH
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Sclerostin (SOST) is also known as Sclerosteosis, VBCH, is a secreted glycoprotein with a signal peptide for secretion and a C-terminal cysteine knot-like (CTCK) domain and belongs to the Cerberus/DAN family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. More recently Sclerostin has been identified as binding to LRP5/6 receptors and inhibiting the Wnt signalling pathway. Wnt pathway inhibition under these circumstances is antagonistic to bone formation (meaning Sclerostin antagonizes bone formation). It has been shown that SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. Sclerostin production by osteocytes is inhibited by parathyroid hormone, mechanical loading and cytokines including oncostatin M, cardiotrophin-1 and leukemia inhibitory factor. Sclerostin production is increased by calcitonin. Thus, osteoblast activity is self regulated by a negative feedback system. Mutations of Sclerostin is associated with the syndrome Sclerosteosis, and reduced sclerostin expression results in a milder form of the disorder called van Buchem disease.
Background References:
- Li X, et al., 2005, J. Biol. Chem. 280 (20): 19883?7.
- Van Bezooijen RL, et al., 2007, J. Bone Miner. Res. 22 (1): 19?28.
- Krause C, et al., 2010, J. Biol. Chem. 285 (53): 41614?26.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
50964
NCBI Official Name:
sclerostin
NCBI Official Symbol:
SOST
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
22.5 kDa
Protein Accession #:
AAI01087.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q9BQB4