anti-Myosin IIA (non-muscle) (heavy chain) mAb (rec.) (SF9) (ATTO 488)

AdipoGen Life Sciences
Product Code: AG-27B-0010TD
Product Group: Primary Antibodies
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AG-27B-0010TD-C100100 ug£300.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Isotype: Human IgG2λ
Antibody Clonality: Monoclonal
Antibody Clone: SF9
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Immunocytochemistry (ICC)
Shipping:
+4°C
Storage:
+4°C

Images

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Further Information

Alternate Names/Synonyms:
Cellular Myosin Heavy Chain, Type A; Myosin Heavy Chain 9; Myosin Heavy Chain, Non-muscle IIa; Non-muscle Myosin Heavy Chain A
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
Full length myosin IIA from rat liver.
Labels - Conjugates:
ATTO 488
Long Description:
Recombinant Antibody. Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain). Species cross-reactivity: Drosophila, Human, Mouse, Rat. Clone: SF9. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
NCBI, Uniprot Number:
P35579
Other data:
anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) (ATTO 488) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain and labeled with ATTO 488.
Package Type:
Plastic Vial
Product Description:
anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Purity:
>95% (SDS-PAGE)
Source / Host:
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Specificity:
Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibody Conjugates
UNSPSC Number:
12352203
Use & Stability:
Keep conjugated formats at +4°C.

References

Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003)