anti-BTLA (human) mAb (6F4)

AdipoGen Life Sciences
Product Code: AG-20B-0049
Product Group: Primary Antibodies

CodeSizePrice
AG-20B-0049-C100100 ug£330.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Clonality: Monoclonal
Antibody Clone: 6F4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Functional Study
Shipping:
-20°C
Storage:
-20°C

Further Information

Alternate Names/Synonyms:
B and T Lymphocyte Associated; Lymphotoxin-beta Receptor; Tumor Necrosis Factor Receptor 2 Related Protein; Tumor Necrosis Factor C Receptor; Tumor Necrosis Factor Receptor Superfamily Member 3; TNFRSF3
Concentration:
1mg/ml
EClass:
32160000
Endotoxin:
<0.001EU/µg purified protein.
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Ectodomain of human BTLA fused to human IgG Fc.
Long Description:
Monoclonal Antibody. Recognizes human BTLA. Isotype: Rat IgG1. Clone: 6F4. Applications: ELISA, FACS, FUNC (Blocking). Liquid. In PBS containing 10% glycerol. B and T lymphocyte associated (BTLA) is an Ig domain superfamily protein with cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. The herpes virus entry mediator (HVEM), a member of the TNF receptor (TNFR) superfamily, can act as a molecular switch that modulates T cell activation by propagating positive signals from the TNF-related ligand LIGHT (TNFR superfamily 14), or inhibitory signals through the Ig superfamily member BTLA. The binding site on HVEM for BTLA is conserved in the orphan TNF receptor UL144, present in human CMV. UL144 binds BTLA, but not LIGHT, and inhibits T cell proliferation, selectively mimicking the inhibitory cosignaling function of HVEM.
NCBI, Uniprot Number:
Q7Z6A9
Package Type:
Plastic Vial
Product Description:
B and T lymphocyte associated (BTLA) is an Ig domain superfamily protein with cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. The herpes virus entry mediator (HVEM), a member of the TNF receptor (TNFR) superfamily, can act as a molecular switch that modulates T cell activation by propagating positive signals from the TNF-related ligand LIGHT (TNFR superfamily 14), or inhibitory signals through the Ig superfamily member BTLA. The binding site on HVEM for BTLA is conserved in the orphan TNF receptor UL144, present in human CMV. UL144 binds BTLA, but not LIGHT, and inhibits T cell proliferation, selectively mimicking the inhibitory function of HVEM. BTLA also interacts with HVEM in cis, forming a heterodimeric complex in naive T cells that inhibits HVEM-dependent signaling. The cis-interaction between HVEM and BTLA is the predominant form expressed on the surface of naive human and mouse T cells and may be important to limit trans-signaling by LIGHT, BTLA, and CD160 that are expressed in adjacent activated cells.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Recognizes human BTLA.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

T Cell Intrinsic Heterodimeric Complexes between HVEM and BTLA Determine Receptivity to the Surrounding Microenvironment: T.C. Cheung, et al.; J. Immunol. 183, 7286 (2009) | Unconventional ligand activation of herpesvirus entry mediator signals cell survival: T.C. Cheung, et al.; PNAS 106, 6244 (2009)