IL-18 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-506-10ug | 10ug | £404.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C.
Documents
Further Information
Additional Names:
Interleukin-18, Interferon-gamma-inducing Factor, IGIF, IL-1gamma, IL1F4
Application Note:
This recombinant proteins is for research use only.
Background:
Interleukin-18 (IL-18) is a costimulatory factor for production of interferon-gamma (IFN-gamma) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE; Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-gamma also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 have also been reported, such as enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF, IFN-gamma in T cells and enhancement of Fas ligand expression by Th1 cells.
Buffer:
Lyophilized from 0.2um-filtered solution in PBS. Reconstituted in sterile H2O not less than 100 μg/ml, which can then be further diluted in other aqueous solutions.
Fusion Tag:
His Tag
NCBI Gene ID #:
3606
NCBI Official Name:
interleukin 18
NCBI Official Symbol:
IL18
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_001553
Protein GI Number:
4504653
Purity:
>97% (SDS-PAGE).
Endotoxin level is less than 1EU/ μg protein (LAL test; Lonza).
Endotoxin level is less than 1EU/ μg protein (LAL test; Lonza).
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q9JI71