CD40L multimeric Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-232-10ug | 10ug | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L
Application Note:
This recombinant proteins is for research use only.
Background:
MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
Biological Activity:
Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).
Buffer:
Contains PBS. Reconstitute with 100 μl sterile water.
Concentration:
0.1mg/ml after reconstitution.
Fusion Tag:
DDDDK Tag
NCBI Gene ID #:
21947
NCBI Official Name:
CD40 ligand
NCBI Official Symbol:
Cd40lg
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P27548
Purity:
>95% (SDS-PAGE).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
Recombinant Protein Sequence:
Thr32-Gly402
Source:
CHO cells
Swissprot #:
P14778