UBE2M Recombinant Protein
| Code | Size | Price |
|---|
| PSI-92-178-0.05mg | 0.05mg | £389.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Documents
Further Information
Additional Names:
NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBC12, UBE2M
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Buffer:
Supplied as a 0.2 um filtered solution of 50mM HEPES,pH 7.5,2mM DTT,150mM NaCl,10%glycerol . It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Fusion Tag:
Tag Free
NCBI Gene ID #:
9040
NCBI Official Name:
ubiquitin conjugating enzyme E2 M
NCBI Official Symbol:
UBE2M
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
20.9 kD
Protein Accession #:
P61081
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P61081