PBEF Recombinant Protein

ProSci
Product Code: PSI-92-711
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-92-711-0.05mg0.05mg£597.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Storage:
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.

Documents

Further Information

Additional Names:
Pre-B cell-enhancing factor, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, PBEF, PBEF1
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine ?visfatin?. PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF- alpha, IL-1 beta , IL-16, and TGF- beta 1. PBEF also increases the production of IL-6, TNF- alpha, and IL-1 beta in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells.
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM HEPES, 150mM NaCl, pH8.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
N-6 His tag
NCBI Gene ID #:
10135
NCBI Official Name:
nicotinamide phosphoribosyltransferase
NCBI Official Symbol:
NAMPT
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
57 kD
Protein Accession #:
P43490
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P43490