IL-36 alpha Recombinant Protein

ProSci
Product Code: PSI-91-587
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-91-587-0.05mg0.05mg£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Mouse
Storage:
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.

Documents

Further Information

Additional Names:
Interleukin-36 alpha, Il36a, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, nterleukin-1 family member 6, IL-1F6, Interleukin-1 homolog 1, IL-1H1, Fil1e, Il1e, Il1f6, Il1h1
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Mouse Interleukin-36 alpha(Il-36a)is a member of the IL-1 family.IL?1 alpha,IL?1 beta and IL-18 are potent inflammatory cytokines whose activities are dependent on heterodimeric receptors of the IL-1R superfamily, and which are regulated by soluble antagonists. Il36a is expressed in many cells, including monocytes, B cells, and T cells, immune system and fetal brains. Il36a is a cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. It is a part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. It seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. It Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs). Moreover, it is involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II and can induce the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T cells and splenocytes. Il36a may play a role in proinflammatory effects in the lung: induces the expression of CXCL1 and CXCL2 in the lung, and the expression of TNF-alpha, IL-36c, IL-1A, IL-1B, CXCL1 and CXCL2 in isolated splenic CD11c+ alveolar macrophages. It may be involved in T cell maturation by stimulating the surface expression of CD40 and modestly CD80 and CD86 in splenic CD11c+ cells and CD4+ T cell proliferation.
Buffer:
Lyophilized from a 0.2 um filtered solution of PBS,pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
Tag Free
NCBI Gene ID #:
54448
NCBI Official Name:
interleukin 1 family, member 6
NCBI Official Symbol:
Il1f6
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
17.27 kD
Protein Accession #:
Q9JLA2
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q9JLA2