anti-RasGRP2 pAb (IG-09)
| Code | Size | Price |
|---|
| AG-25T-0118-R100 | 100 ul | £380.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Application: Western Blot (WB)
Shipping:
+4°C
Storage:
Short Tem: +4°C Long Term: +4°C
Documents
Further Information
Alternate Names/Synonyms:
CalDAG-GEFI; Ras Guanyl Nucleotide-releasing Protein 2; Ras Guanyl Releasing Protein 2; CDC25-like Protein; CDC25L; Calcium and DAG-regulated Guanine Nucleotide Exchange Factor I; MCG7
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 2mg/ml BSA and 0.05% sodium azide.
Handling Advice:
Do not freeze.Keep sterile.
Immunogen:
Synthetic peptide corresponding to a human RasGRP2 sequence located at the C-terminus of the RasGEF domain.
Long Description:
Polyclonal Antibody. Recognizes human and mouse RasGRP2. Source: Rabbit. Applications: WB. Liquid. In PBS containing 2mg/ml BSA and 0.05% sodium azide. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.
NCBI, Uniprot Number:
Q7LDG7
Package Type:
Plastic Vial
Product Description:
RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.
Purity:
Antigen affinity purified.
Source / Host:
Rabbit
Specificity:
Recognizes human and mouse RasGRP2.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 6 months after receipt when stored at +4°C.
References
Phosphorylation of CalDAG-GEFI by protein kinase A prevents Rap1b activation: H. Subramanian, et. al.; Thromb. Haemost. 11, 1574 (2013)