Clusterin (secretory form) (human) (rec.)

AdipoGen Life Sciences
Product Code: AG-40A-0050Y
Product Group: Recombinant Proteins

CodeSizePrice
AG-40A-0050Y-C01010 ug£160.00
Quantity:
AG-40A-0050Y-C05050 ug£260.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Human
Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
Short Tem: +4°C, Long Term: -20°C

Images

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After denaturation of recombinant secretory clusterin in the presense of beta-mercaptoethanol followed by boiling, two distinct bands, 60kDa and 35kDa, were seperated on SDS-PAGE. The two bands were extracted and subjected to determination of their N-te

After denaturation of recombinant secretory clusterin in the presense of beta-mercaptoethanol followed by boiling, two distinct bands, 60kDa and 35kDa, were seperated on SDS-PAGE. The two bands were extracted and subjected to determination of their N-te

Further Information

Alternate Names/Synonyms:
TRPM-2; Apolipoprotein J; APO-J; CLI; CLU; SGP-2
Concentration:
After reconstitution:for 10µg size: 0.1mg/mlfor 50µg size: 1mg/ml
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. Signal peptide sequence and the human secretory clusterin (aa 1-449) are fused at the C-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Purity: >90% (SDS-PAGE). Clusterin shares homology with the small heat shock protein family of molecular chaperones. The mature secreted form of the protein is a glycosylated, 80kDa disulfide-linked heterodimer of alpha and beta subunits (produced by internal cleavage). Clusterin is expressed in virtually all tissues and found in all human fluids. It is involved in numerous physiological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling and immune system regulation. Clusterin also exists as a nuclear protein. The secreted form of Clusterin has extracellular chaperone and anti-apoptotic activities while the nuclear form acts as a proapoptotic factor.
Molecular Weight:
80kDa (under non-reducing conditions); 39kDa and 40kDa (under reducing conditions) (by SDS-PAGE).
NCBI, Uniprot Number:
P10909
Package Type:
Plastic Vial
Product Description:
Clusterin shares homology with the small heat shock protein family of molecular chaperones. The mature secreted form of the protein is a glycosylated, 80kDa disulfide-linked heterodimer of alpha and beta subunits (produced by internal cleavage). Clusterin is expressed in virtually all tissues and found in all human fluids. It is involved in numerous physiological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling and immune system regulation. Clusterin also exists as a nuclear protein. The secreted form of Clusterin has extracellular chaperone and anti-apoptotic activities while the nuclear form acts as a proapoptotic factor.
Purity:
>90% (SDS-PAGE)
Sequence:
Signal peptide sequence and the human secretory clusterin (aa 1-449) are fused at the C-terminus to a FLAG®-tag.
Source / Host:
HEK 293 cells
TAGs:
FLAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

References

Clusterin induces matrix metalloproteinase-9 expression via ERK1/2 and PI3K/Akt/NF-kappaB pathways in monocytes/macrophages: Y.-J. Shim, et al.; J. Leuk. Biol. 90, 761 (2011) | Clusterin and LRP2 are critical components of the hypothalamic feeding regulatory pathway: S.Y. Gil, et al.; Nat. Commun. 4, 1862 (2013) | Clusterin/ApoJ enhances central leptin signaling through Lrp2-mediated endocytosis: K. Byun, et al.; EMBO Rep. 15, 801 (2014)