CD137L Soluble (human) (rec.)
| Code | Size | Price |
|---|
| AG-40A-0198T-C050 | 50 ug | £330.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
Short Term: +4°C. Long Term: -20°C
Further Information
Alternate Names/Synonyms:
4-1BBL; TNFSF9
Concentration:
1mg/ml after reconstitution
EClass:
32160000
Endotoxin:
<0.06EU/µg purified protein (LAL test; Lonza).
Form (Short):
solid
Formulation:
Lyophilized from 0.2µm-filtered solution in PBS, pH 7.2.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. The extracellular domain of human CD137L (aa 50-254) is fused at the N-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.06EU/µg purified protein (LAL test; Lonza). Lyophilized from 0.2µm-filtered solution in PBS, pH 7.2. Purity: >90% (SDS-PAGE). The CD137 ligand (CD137L; 4-1BBL) is a member of the tumor necrosis factor (TNF) family. CD137L is a type II transmembrane protein found on activated macrophages, dendritic cells and mature B cells. The interaction with its receptor 4-1BB induces recruitment of TNF receptor-associated factor 1 (TRAF1) and TRAF2 and interaction with the kinase p56lck. CD137 and CD137L have been reported to be involved in tumor rejection, apoptosis, anti-viral immunity, diabetes, in T and B cell co-stimulation and modulation of the immune response.
Molecular Weight:
~23kDa (SDS-PAGE)
NCBI, Uniprot Number:
P41273
Package Type:
Plastic Vial
Product Description:
The CD137 ligand (CD137L; 4-1BBL) is a member of the tumor necrosis factor (TNF) family. CD137L is a type II transmembrane protein found on activated macrophages, dendritic cells and mature B cells. The interaction with its receptor 4-1BB induces recruitment of TNF receptor-associated factor 1 (TRAF1) and TRAF2 and interaction with the kinase p56lck. CD137 and CD137L have been reported to be involved in tumor rejection, apoptosis, anti-viral immunity, diabetes, in T and B cell co-stimulation and modulation of the immune response.
Purity:
>90% (SDS-PAGE)
Sequence:
The extracellular domain of human CD137L (aa 50-254) is fused at the N-terminus to a FLAG®-tag.
Source / Host:
HEK 293 cells
Specificity:
Binds to human CD137.
TAGs:
FLAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
References
Production of recombinant human trimeric CD137L (4-1BBL). Cross-linking is essential to its T cell costimulationactivity: C. Rabu, et al.; J. Biol. Chem. 280, 41472 (2005) | A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade: C. Geuijen, et al.; Nature Commun. 12, 4445 (2021)