UBE2D3 [UbcH5c ] (human) (rec.) (untagged)

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South Bay Bio
Product Code: SBB-CE0019
Product Group: Recombinant Proteins
Supplier: South Bay Bio
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SBB-CE0019-C100100 ug£336.00
Quantity:
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Overview

Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-80C

Images

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Thioester Activity Assay: UBE2D3 (UbcH5c) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D3.
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Thioester Activity Assay: UBE2D3 (UbcH5c) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D3.

Documents

Further Information

Alternate Names/Synonyms:
Ubiquitin-conjugating Enzyme H5c; PUBC; UBC5C; Ubiquitin-protein Ligase D3; p53-regulated Ubiquitin-conjugating Enzyme
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM HEPES pH 8, 200mM sodium chloride, 10% glycerol (v/v), 1mM TCEP.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Protein. Human UBE2D3 (aa1-147)is untagged. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 8, 200mM sodium chloride, 10% glycerol (v/v), 1mM TCEP. Purity: >95% (SDS-PAGE). UBE2D3 (UbcH5c) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D3 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D3-Ub thioester. UBE2D3 is capable of associating with numerous known E3 ligases including MDM2, which target proteins such as p53 for proteasomal degradation through polyubiquitination.
Molecular Weight:
~17kDa
NCBI, Uniprot Number:
P61077
Package Type:
Plastic Vial
Product Description:
UBE2D3 [UbcH5c] is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D3 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D3-Ub thioester. UBE2D3 is capable of associating with numerous known E3 ligases including MDM2, which target proteins such as p53 for proteasomal degradation through polyubiquitination.
Purity:
>95% (SDS-PAGE)
Sequence:
Human UBE2D3 (aa1-147) (Accession Nr. P61077 http://www.ncbi.nlm.nih.gov/protein/P61077 ) is untagged.
Source / Host:
E. coli
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.