UBE2L3 [UbcH7] (human) (rec.) (untagged)
Code | Size | Price |
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SBB-CE0020-C100 | 100 ug | £336.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-80C
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Further Information
Alternate Names/Synonyms:
Ubiquitin-conjugating Enzyme E2 L3; E2-F1; L-UBC; UBCE7; UbcM4; UBE2L3; Ubiquitin-protein Ligase L3
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Protein. Human UBE2L3 (aa 1-154)is untagged. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP. Purity: >95% (SDS-PAGE). UBE2L3 (UbcH7) is an E2 ubiquitin conjugating enzyme and accepts ubiquitin from an E1 activating enzyme via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2L3-Ub thioester. UBE2L3 transfers active ubiquitin molecules via a final transthiolation reaction to a cysteine residue of HECT class or RBR class E3 ligases. UBE2L3 plays a critical role in the activation of NF-kappab signaling by working in conjugation with the RBR E3 ligase, LUBAC (linear ubiquitin assembly complex), to ubiquitinate NEMO of the IkappaB complex, which than facilitates the downstream immune response.
Molecular Weight:
~18kDa
NCBI, Uniprot Number:
P68036
Package Type:
Plastic Vial
Product Description:
UBE2L3 (UbcH7) is an E2 ubiquitin conjugating enzyme and accepts ubiquitin from an E1 activating enzyme via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2L3-Ub thioester. UBE2L3 transfers active ubiquitin molecules via a final transthiolation reaction to a cysteine residue of HECT class or RBR class E3 ligases. UBE2L3 plays a critical role in the activation of NF-kappab signaling by working in conjugation with the RBR E3 ligase, LUBAC (linear ubiquitin assembly complex), to ubiquitinate NEMO of the IkappaB complex, which than facilitates the downstream immune response.
Purity:
>95% (SDS-PAGE)
Sequence:
Human UBE2L3 (aa 1-154) (Accession Nr. P68036 http://www.ncbi.nlm.nih.gov/protein/P68036 ) is untagged.
Source / Host:
E. coli
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.