PLpro (SARS Coronavirus) (rec.) (His)

South Bay Bio
Product Code: SBB-DE0024
Product Group: Recombinant Proteins
Supplier: South Bay Bio

CodeSizePrice
SBB-DE0024-C05050 ug£275.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-80C

Further Information

Alternate Names/Synonyms:
Papain-like Proteinase; SARS Coronavirus Main Proteinase; Non-structural Protein 3; PL2-PRO; Nsp3
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Protein. PLpro (Papain-like Protease) (aa 1540-1841)is fused at the N-terminus to a His-tag. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP. Purity: >95% (SDS-PAGE). The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) is involved in the processing of the viral polyprotein. Proteolytic processing of the coronavirus replicase poly-protein is essential for generating a functional virus replication complex. PLpro possesses both deubiquitinating or deISGylating activity and can process Lys48 and Lys63 linked polyubiquitin chains (free chains or from cellular substrates). It works in concert together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. It strongly antagonizes the innate immune induction of type I interferon by blocking the phosphorylation, dimerization and therefore the nuclear translocation of host IRF3. In addition, it prevents also host NF-kappa-B signaling.
Molecular Weight:
~37kDa
NCBI, Uniprot Number:
P0C6U8
Package Type:
Plastic Vial
Product Description:
The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) is involved in the processing of the viral polyprotein. Proteolytic processing of the coronavirus replicase poly-protein is essential for generating a functional virus replication complex. PLpro possesses both deubiquitinating or deISGylating activity and can process Lys48 and Lys63 linked polyubiquitin chains (free chains or from cellular substrates). It works in concert together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. It strongly antagonizes the innate immune induction of type I interferon by blocking the phosphorylation, dimerization and therefore the nuclear translocation of host IRF3. In addition, it prevents also host NF-kappa-B signaling.
Purity:
>95% (SDS-PAGE)
Sequence:
PLpro (Papain-like Protease) (aa 1540-1841) (Accession Nr. P0C6U8 http://www.ncbi.nlm.nih.gov/protein/P0C6U8 ) is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.