NEDP1 [SENP8] (human) (rec.) (His)

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South Bay Bio
Product Code: SBB-DE0025
Product Group: Recombinant Proteins
Supplier: South Bay Bio
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SBB-DE0025-C05050 ug£244.00
Quantity:
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Overview

Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-80C

Images

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Michaelis?Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125?M was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25?C. Initial velocities a
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Michaelis?Menten Kinetics: NEDD8 Rhodamine 110 serially diluted from 1.6 to 0.0125?M was digested with 30pM NEDP1 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 1mM TCEP, 0.1 mg/ml BSA, at 25?C. Initial velocities a

Documents

Further Information

Alternate Names/Synonyms:
NEDD8-specific Protease 1; Sentrin-specific Protease 8; Deneddylase-1; Sentrin/SUMO-specific Protease SENP8; DEN1; PRSC2
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Protein. Human NEDP1 (aa2-212)is fused at the N-terminus to a His-tag. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP. Purity: >95% (SDS-PAGE). NEDP1 is a deconjugating enzyme with specificity towards NEDDylated substrates. NEDP1 discriminates between ubiquitinated and NEDDylated targets, despite similar C-terminal regions. NEDP1 is a protease which hydrolyzes the isopeptide bond between the C-terminus of NEDD8 and the respective lysine of the substrate protein to which the NEDD8 is covalently bound. NEDP1 is important in regulating cullin associated ubiquitin ligase enzymes such as those of the SCF (Skp-Cullin-Fbox) family and subsequently, it is important in regulating cell cycle progression and other biological functions.
Molecular Weight:
~27kDa
NCBI, Uniprot Number:
Q96LD8
Package Type:
Plastic Vial
Product Description:
NEDP1 is a deconjugating enzyme with specificity towards NEDDylated substrates. NEDP1 discriminates between ubiquitinated and NEDDylated targets, despite similar C-terminal regions. NEDP1 is a protease which hydrolyzes the isopeptide bond between the C-terminus of NEDD8 and the respective lysine of the substrate protein to which the NEDD8 is covalently bound. NEDP1 is important in regulating cullin associated ubiquitin ligase enzymes such as those of the SCF (Skp-Cullin-Fbox) family and subsequently, it is important in regulating cell cycle progression and other biological functions.
Purity:
>95% (SDS-PAGE)
Sequence:
Human NEDP1 (aa2-212) (Accession Nr. Q96LD8 http://www.ncbi.nlm.nih.gov/protein/Q96LD8 ) is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.