Asprosin (human) Matched Pair Detection Set

Fibrillin-1 C-terminal Cleavage Product; FBN1 C Terminal Cleavage Product

Sandwich

Colorimetric

32160000

Avoid freeze/thaw cycles.

1 vial standard protein (100ng) (lyophilized) [STD] | 1 vial coating antibody (200µl) [COAT] | 1 vial detection antibody (60µl) [DET] | 1 vial streptavidin-HRP (10µg) (lyophilized) [STREP]

Matched Pair Detection Set. Detects human Asprosin in cell culture supernatant. Assay Type: Sandwich. Detection Type: Colorimetric. Sample Type: Cell Culture Supernatant. Range: 0.156 to 10ng/nl. Sensitivity: 150pg/ml. Asprosin is a new fasting-induced protein hormone that targets the liver to increase plasma glucose levels. Asprosin is the C-terminal cleavage product of the protein fibrillin-1. Asprosin is secreted from white adipose tissue and increases hepatic glucose production by using cAMP as a second messenger, leading to activation of protein kinase A in the liver. Reduction of asprosin levels protect against metabolic syndrome-associated hyperinsulinism.

Box

Asprosin is a new fasting-induced protein hormone that targets the liver to increase plasma glucose levels. Asprosin is the C-terminal cleavage product of the protein pro-Fibrillin-1. Asprosin is secreted from white adipose tissue and increases hepatic glucose production by using cAMP as a second messenger, leading to activation of protein Kinase A. Reduction of Asprosin levels protects against metabolic syndrome-associated hyperinsulinism. Asprosin may act as a circulating hunger signal. Indeed, peripherally injected recombinant asprosin can cross the blood-brain barrier and intracerebroventricular (i.c.v.) injection of recombinant asprosin stimulated appetite in wild-type mice, indicating a central mechanism of action. Loss of Asprosin in mice and human leads to decreased fat mass and body weight, and hypophagia. Mice are also completely protected from the development of diet-induced obesity. Asprosin works by stimulating the orexigenic AgRP+ (Agouti related neuropeptide) neurons via a cAMP-dependent pathway and by inhibiting the anorexigenic neurons POMC+ (pro-opiomelanocortin) neurons in a GABA-dependent manner. Mutation in Asprosin in human leads to the pattern of metabolic dysregulation, including partial lipodystrophy, accompanied by reduced plasma insulin. Due to its key role in food regulation, Asprosin function could serve as a potentially unique therapeutic target against obesity, diabetes or metabolic diseases.

0.156 to 10ng/ml

Cell Culture Supernatant

150pg/ml

Detects human Asprosin in cell culture supernatant.

Non-hazardous

ELISA Kits

41116126

Stable for at least 1 year after receipt when stored at -20°C.