G-CSF Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-785-0.05mg | 0.05mg | £898.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
G-CSF,CSF3,C17orf33,CSF3OS,MGC45931
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
Granulocyte colony-stimulating factor (G-CSF or GCSF) is also known as colony-stimulating factor 3, CSF3, C17orf33, CSF3OS, GCSF, MGC45931. It is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates them using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras /mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. The natural human glycoprotein exists in two forms, a 174- and 180-amino-acid-long protein of molecular weight 19,600?grams per mole. G-CSF can effect on the hematopoietic system and neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis. G-CSF stimulates the production of white blood cells (WBC). In oncology and hematology, a recombinant form of G-CSF is used with certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells). The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim.
Background References:
- Schneider A,, et al., 2005, J. Clin. Invest. 115 (8): 2083?98.
- Pitzer C, et al., 2008, Brain 131 (Pt 12): 3335?47.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human G-CSF, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human G-CSF R, His Tag with a linear range of 0.156-1.25 ug/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
Tag Free
NCBI Gene ID #:
1440
NCBI Official Name:
granulocyte colony-stimulating factor isoform b precursor
NCBI Official Symbol:
G-CSF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
18.7 kDa
Protein Accession #:
NP_757373.1
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P09919