IL-10 Recombinant Protein

ProSci
Product Code: PSI-97-003
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-97-003-0.02mg0.02mg£469.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.

Documents

Further Information

Additional Names:
CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Interleukin-10 (IL-10) is also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Mature human IL-10 shares 72% - 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. Knockout studies suggested the function of Interleukin-10 / IL-10 as an essential immunoregulator in the intestinal tract. Patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin-10, showing the importance of interleukin-10 for counteracting excessive immunity in the human body.
Background References:
  • Vieira, P. et al., 1991, Proc. Natl. Acad. Sci. 88:1172.
  • Hsu, D.H. et al., 1990, Science 250:830.
  • Sabat, R. et al., 2010, Cytokine Growth Factor Rev. 21:331.
  • Blackburn, S.D. and E.J. Wherry, 2007, Trends Microbiol. 15:143.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
16153
NCBI Official Name:
interleukin-10 precursor
NCBI Official Symbol:
IL-10
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
19.6 kDa
Protein Accession #:
NP_034678
Purity:
>92% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P18893