FOLR1 Recombinant Protein

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ProSci
Product Code: PSI-96-795
Product Group: Recombinant Proteins
Supplier: ProSci

CodeSizePrice
PSI-96-795-0.1mg0.1mg£867.00
Quantity:
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Overview

Regulatory Status: RUO
Target Species:
  • Cynomolgus Monkey
  • Rhesus Macaque
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.

Documents

Further Information

Additional Names:
FOLR-1,FBP,FOLR
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
Background References:
  • Lacey SW, et al., 1989, J. Clin. Invest. 84 (2): 715?20.
  • Elwood PC, 1989, J. Biol. Chem. 264 (25): 14893?901.
  • Rijnboutt, S. et al., 1996, J. Cell Biol. 132:35.
  • Parker, N. et al., 2005, Anal. Biochem. 338:284.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Folic acid-BSA conjugate at 20 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque FOLR1, His Tag with a linear range of 0.156-2.5 ug/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
718388
NCBI Official Name:
folate receptor alpha precursor
NCBI Official Symbol:
FOLR1
NCBI Organism:
Macaca fascicularis?
Physical State:
Lyophilized
Predicted Molecular Weight:
26.4 kDa
Protein Accession #:
NP_001181576.1
Purity:
>90% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
G7PR14-1